|
|
|
|
|
|
|
|
|
|
| PARP LAB PROTOCOLS |
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
| |
|
|
|
Home | Information Center | Community | Resources | Protocols | News | Contributors | Contacts |
|
PARP-1 exon 4 KO mice typing
contributed by Josiane Ménissier-de Murcia (Illkirch, France)
|
Primers :
| OLIGO (+) |
GGCCAGATGCGCCTGTCCAAGAAG |
| OLIGO (-) |
GGCGAGGATCTCGTCGTGACCCAT |
| OLIGO (+/-) |
CTTGATGGCCGGGAGCTGCTTCTTC |
PCR conditions :
Standard PCR buffer conditions and Taq-DNA polymerase
| First step |
2 min at 94°C |
| 30 cycles |
40 sec at 94°C |
|
45 sec at 61°C |
|
1 min at 73°C |
| Final step |
4 min at 73°C |
Expected PCR products :
| • wild type allele |
oligo(+/-) and oligo (+) combination |
200 base pairs |
| • mutated allele |
oligo(+/-) and oligo (-) combination |
700 base pairs |
Reference : unpublished
|
|
|
The PARP Link © 2002-2009 - IREBS at the ESBS - CNRS - ESBS - Illkirch - France
|
