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A Web resource on the PARP protein family and poly(ADP-ribosyl)ation
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Protocol index

Biochemistry & Enzymology

PARP activity in cells

Affinity purification of recombinant PARPs
PARP activity in solution

PARP activity blot

Preparation of activated DNA

Zinc binding blot

Southwestern and footprint

NAD assay in cells

PARP activity monitored by NAD(P)H depletion

Base excision repair assay

XRCC1 recruitment


Cell Biology

Antipolymer H10 hybridoma

• Immunocytochemistry :
1) poly(ADP-ribose) synthesis
2) PARP activation in oxidatively stressed cells
3) immunocytochemical staining of poly(ADP-ribose)

PARP-1 KO mice typing

PARP-2 KO mice typing

Mouse embryonic fibroblasts (MEF) cell lines

• primary cell lines [In preparation]

• immortalized cell lines [In preparation]






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