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PARP proteins & antibodies (continued)

ANTIBODIES

Several antisera and monoclonal antibodies are commercially available from different companies (Alexis Biochemicals , BioMol,
Calbiochem, Roche, Serotec, R&D Systems, Trevigen and others).

ANTISERA
SPECIFICITY
USED FOR
@ bovine PARP-1 human, bovine, monkey, mouse, rat PARP-1
IP, WB, IF
@ PARP-1 (human recombinant) human, bovine, chicken, mouse, xenopus PARP-1
IF, WB
@ PARP-1 (automodification domain) human, bovine, hamster, monkey, mouse, rat PARP-1
IP, WB
@ PARP-1 (short, aa 82-96 in mouse) human and mouse short PARP-1 and PARP-1
IF, WB
@ human recombinant PARP-1 (aa 1-232) PARP-1 & the apoptosis-induced cleavage fragment
EL, IP, WB, IF
@ human recombinant PARP-1 (aa 337-573) PARP-1 & the apoptosis-induced cleavage fragment
EL, IP, WB, IF
@ human PARP-1 (synth., aa 197-214) PARP-1 & the apoptosis-induced cleavage fragment
EL, IP, WB, IF
@ human PARP-1 (synth., aa 215-228) PARP-1 & the apoptosis-induced cleavage fragment
EL, WB, IF
@ human PARP-1 (synth., aa 214-215) N-terminus of the cleavage site (214-215) of hPARP-1
WB, IF
@ human PARP-1/FITC (synth., aa 214-215) N-terminus of the cleavage site (214-215) of hPARP-1
Flow Cyt.
@ bovine PARP-1 (aa 509-524) human, bovine, rodent PARP & the caspase cleavage fragment
WB
@ mouse recombinant PARP-2 human, mouse or monkey PARP-2
EL, IP, WB, IF
@ human VPARP (PARP-4) (aa 24-38) human Vault PARP (PARP-4)
WB
@ poly(ADP-ribose) poly(ADP-ribose) synthesized in vivo or in vitro
EL, IF, IP, WB
MONOCLONAL ANTIBODIES
SPECIFICITY
USED FOR
@ PARP-1 Mab (53015.11) immunogen : mouse (aa 71-329) ; recognizes human and mouse PARP-1
EL, IF, IP, WB
@ PARP-1 Mab (C2-10) recognizes both the full length & the 85 kDa cleavage fragment of PARP-1 in human, monkey, mouse, rat, bovine and hamster systems. Chicken PARP is not recognized
EL, IF, IP, WB
@ PARP-1 Mab (F1-23) recognizes the DBD of PARP-1 & the apoptosis-induced cleavage fragment. Does not recognize rodent PARP-1
EL, IF, IP, WB
@ poly (ADP-ribose) (PAR) Mab (10H) poly(ADP-ribose) synthesized in vivo or in vitro
Hybridoma available from
RIKEN Cell Bank in Japan (Cell No.: RCB0705, RCB1142)
WB
@ tankyrase (PARP-5) Mab (19A449) human tankyrase
WB
Codes : @: anti- ; IP: immunoprecipitation ; EL: Elisa ; IF: immunofluorescence ; WB: Western blot

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